![]() ![]() Some paramecia were lost in the shown analysis. It looks like I should have set the circularity=0.3-1.00 insetad of 0.5-10.00. Carefully optimize the ragne of areas and circularity so that paramecia can be detected. Averaging a longer movie gives a better result. Run(“Analyze Particles…”, “size=15-90 circularity=0.50-1.00 show=Masks display clear summarize stack”) Ĭlick “Run” to close all the image windows.īe aware of the errors in this method of counting paramecia. Note: Later I noticed that circularity=0.30-1.00 may work beter. Run(“Enhance Contrast”, “saturated=0.35”) Īdjust threshold min and max and “Apply”, and click “OK”. ImageCalculator(“Divide create 32-bit stack”, image1,image2) Here I have dozens of short movies (~ 1 sec) for which I want to count the number of paramecia.īefore going through the following steps, prepare the Macros by copy and paste. To obtain multiple tabs, in the Macro window, go to File > New, then go to Language > IJ1 Macro. Copy and paste each block into each tab of the Macro window. Here is a macro that can be used for the sample movie, to reproduce the following procedure by yourself. Here is an example of what I have done recently.įirs, make macro windows by going to Plugins > New > Macro. To run Macro recording, Go to Plugin > Macros > Record… Use Macro recording to generate macro while you do what you want to do in the macro. In such a case, running peices of macro commands one by one, while manually working on critical steps may help.įirst of all, get Fiji/ImageJ if you don’t have it yet. Yet, it is torturous to move around the mouse and clicking it so many times. When the number of the files to be processed is not extremely large, doing it manually can be quicker than writing a macro that works on any recorded files.
0 Comments
Leave a Reply. |